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The 25??L PCR reaction volumes had been 50 mM KCl, 10 mM Tris?HCl…

The 25??L PCR reaction volumes had been 50 mM KCl, 10 mM Tris?HCl… The 25??L PCR reaction volumes had been 50 mM KCl, 10 mM Tris?HCl, 2.5 mM MgCl2, 0.2 mM each dNTP, 0.2 ?M forward primer, 0.2 ?M reverse primer, 0.05 units/?L LGC Biotecnologia Taq DNA Polymerase, and included about 5–10 ng of genomic